anti perk  (Bioss)

Journal: Cell Death Discovery

Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation

doi: 10.1038/s41420-025-02539-0

Figure Lengend Snippet: A The Venn diagram of common DEGs among the three datasets. B the bar chart of KEGG pathway analysis, indicating that these DEGs are primarily enriched in the Apoptosis, Protein processing in the endoplasmic reticulum, and ECM-receptor interaction pathways. C The GSVA enrichment analysis revealed that the GO-BP of these differentially expressed genes is mainly enriched in endoplasmic reticulum stress. D Representative electron microscopy images of the nucleus pulposus tissue from the NC and IVDD groups. E the volcano plot of these DEGs, showing upregulated expression of PERK and NOXA. F the PPI network diagram of the top 10 key genes, placing PERK and NOXA at the core. G Western blot analysis and quantitative statistical analysis of PERK and NOXA proteins in the NC and IVDD groups. GAPDH was used as an internal control. Data are presented as mean ± SD, ** P < 0.01, *** P < 0.001.

Article Snippet: First antibodies were added, including NOXA (ab222852, 1:100, Abcam, Cambridge, UK), PERK (bs-2469R, 1:50, Bioss, Beijing, China), MMP13 (18165-1-AP, 1:50, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:50, Bioss, Beijing, China), and incubated overnight in a 4 °C refrigerator.

Techniques: Electron Microscopy, Expressing, Western Blot, Control

Journal: Cell Death Discovery

Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation

doi: 10.1038/s41420-025-02539-0

Figure Lengend Snippet: A , B Western blot analysis of the expression levels of apoptosis-related proteins PERK, MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. C , D Representative images of TUNEL staining and quantitative statistical analysis showing the proportion of TUNEL-positive cells in each group. The scale bar is 100 μm. E , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G mRNA levels of PERK, NOXA, BAX, and MMP13 in NPCs from each group. H , I Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: First antibodies were added, including NOXA (ab222852, 1:100, Abcam, Cambridge, UK), PERK (bs-2469R, 1:50, Bioss, Beijing, China), MMP13 (18165-1-AP, 1:50, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:50, Bioss, Beijing, China), and incubated overnight in a 4 °C refrigerator.

Techniques: Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane

Journal: Cell Death Discovery

Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation

doi: 10.1038/s41420-025-02539-0

Figure Lengend Snippet: A Schematic diagram of the overall process of animal experiments. B – D Representative X-ray, Micro-CT, and MRI T2WI images of rats in each group. E Percentage change in disc height index (DHI) of the tail vertebrae in each group of rats. F Histogram of the average gray values of the tail intervertebral discs in each group of rats. G , H Representative images and histological grade of H&E, Safranin O, and Alcian blue staining of the tail intervertebral disc tissue in each group. The scale bar is 500 μm. I , J Representative images and quantitative analysis histogram of immunohistochemical staining for PERK, NOXA, BAX, and ACAN in the NP tissue of rats from different treatment groups. The scale bar is 100 μm. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: First antibodies were added, including NOXA (ab222852, 1:100, Abcam, Cambridge, UK), PERK (bs-2469R, 1:50, Bioss, Beijing, China), MMP13 (18165-1-AP, 1:50, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:50, Bioss, Beijing, China), and incubated overnight in a 4 °C refrigerator.

Techniques: Micro-CT, Staining, Immunohistochemical staining

Journal: Cell Death Discovery

Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation

doi: 10.1038/s41420-025-02539-0

Figure Lengend Snippet: TG induces ERS and leads to apoptosis of NPCs and ECM degradation through the PERK/NOXA/MCL-1 axis. Created in https://BioRender.com .

Article Snippet: First antibodies were added, including NOXA (ab222852, 1:100, Abcam, Cambridge, UK), PERK (bs-2469R, 1:50, Bioss, Beijing, China), MMP13 (18165-1-AP, 1:50, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:50, Bioss, Beijing, China), and incubated overnight in a 4 °C refrigerator.

Techniques:

Journal: Cell death discovery

Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation.

doi: 10.1038/s41420-025-02539-0

Figure Lengend Snippet: Fig. 2 The ERS inhibitor 4-PBA alleviates TG-induced apoptosis and ECM degradation. A, B Western blot analysis of the expression levels of apoptosis-related proteins PERK, MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. C, D Representative images of TUNEL staining and quantitative statistical analysis showing the proportion of TUNEL- positive cells in each group. The scale bar is 100 μm. E, F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G mRNA levels of PERK, NOXA, BAX, and MMP13 in NPCs from each group. H, I Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: First antibodies were added, including NOXA (ab222852, 1:100, Abcam, Cambridge, UK), PERK (bs-2469R, 1:50, Bioss, Beijing, China), MMP13 (18165-1-AP, 1:50, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:50, Bioss, Beijing, China), and incubated overnight in a 4 °C refrigerator.

Techniques: Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane

Journal: Cell death discovery

Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation.

doi: 10.1038/s41420-025-02539-0

Figure Lengend Snippet: Fig. 3 PERK inhibitor GSK rescues NPCs apoptosis and ECM degradation induced by NOXA overexpression. A, B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs GAPDH was used as an internal control. C, E Representative images of TUNEL staining and quantitative statistical analysis showed each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D, F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G, H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I, J Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: First antibodies were added, including NOXA (ab222852, 1:100, Abcam, Cambridge, UK), PERK (bs-2469R, 1:50, Bioss, Beijing, China), MMP13 (18165-1-AP, 1:50, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:50, Bioss, Beijing, China), and incubated overnight in a 4 °C refrigerator.

Techniques: Over Expression, Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane, Cytometry

Journal: Cell death discovery

Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation.

doi: 10.1038/s41420-025-02539-0

Figure Lengend Snippet: Fig. 4 NOXA knockout rescues TG-induced apoptosis and ECM degradation. A, B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs, GAPDH was used as an internal control. C, F Representative images of TUNEL staining and quantitative statistical analysis show each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D, E Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G, H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I, J Representative images and quantitative statistical analysis of the expression of NOXA, BAX, ACAN, and MMP3 in each group by cellular immunofluorescence. The scale bar is 100 μm. K, L Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: First antibodies were added, including NOXA (ab222852, 1:100, Abcam, Cambridge, UK), PERK (bs-2469R, 1:50, Bioss, Beijing, China), MMP13 (18165-1-AP, 1:50, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:50, Bioss, Beijing, China), and incubated overnight in a 4 °C refrigerator.

Techniques: Knock-Out, Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane, Cytometry

Journal: Cell death discovery

Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation.

doi: 10.1038/s41420-025-02539-0

Figure Lengend Snippet: Fig. 5 Imaging and histological analysis of rat coccygeal vertebrae tissue 8 weeks after NOXA gene knockout. A Schematic diagram of the overall process of animal experiments. B–D Representative X-ray, Micro-CT, and MRI T2WI images of rats in each group. E Percentage change in disc height index (DHI) of the tail vertebrae in each group of rats. F Histogram of the average gray values of the tail intervertebral discs in each group of rats. G, H Representative images and histological grade of H&E, Safranin O, and Alcian blue staining of the tail intervertebral disc tissue in each group. The scale bar is 500 μm. I, J Representative images and quantitative analysis histogram of immunohistochemical staining for PERK, NOXA, BAX, and ACAN in the NP tissue of rats from different treatment groups. The scale bar is 100 μm. Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: First antibodies were added, including NOXA (ab222852, 1:100, Abcam, Cambridge, UK), PERK (bs-2469R, 1:50, Bioss, Beijing, China), MMP13 (18165-1-AP, 1:50, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:50, Bioss, Beijing, China), and incubated overnight in a 4 °C refrigerator.

Techniques: Imaging, Gene Knockout, Micro-CT, Staining, Immunohistochemical staining